کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1995329 | 1064962 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Myoendothelial gap junction frequency does not account for sex differences in EDHF responses in rat MCA
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Membrane potential in endothelium was measured using the voltage-sensitive dye di-8-ANEPPS and in smooth muscle using intracellular glass microelectrodes in the presence of l-NAME (3 Ã 10â 5 M) and indomethacin (10â 5 M). Electron microscopy was used to assess MEGJ characteristics. In endothelial cells, the di-8-ANEPPS fluorescence ratio change to 10â 5 M UTP was similar in males (â 2.9 ± 0.5%) and females (â 3.2 ± 0.2%), indicating comparable degrees of endothelial cell hyperpolarization. However, smooth muscle cell hyperpolarization to 10â 5 M UTP was significantly attenuated in females (0 mV hyperpolarization; â 31 ± 1.5 mV resting) compared to males (8 mV hyperpolarization; â 28 ± 1.7 mV resting). Ultrastructural evidence suggested that MEGJ frequency and area of contact were comparable between males and females. Taken together, our data suggest that in rat MCA, MEGJ frequency does not account for the reduced EDHF responses observed in females compared to males. We conclude that reduced myoendothelial coupling and/or homocellular coupling within the media may account for these differences.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 74, Issue 1, July 2007, Pages 39-44
Journal: Microvascular Research - Volume 74, Issue 1, July 2007, Pages 39-44
نویسندگان
Elke M. Sokoya, Alan R. Burns, Sean P. Marrelli, Jie Chen,