کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
19959 1416562 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Recovery of laccase from processed Hericium erinaceus (Bull.:Fr) Pers. fruiting bodies in aqueous two-phase system
چکیده انگلیسی


• Laccase was successful recovered from processed H. erinaceus in PEG/Salt ATPS.
• Cold-stored of H. erinaceus recorded highest laccase activities (2.02 ± 0.04 U/mL).
• Recovery conditions of laccase in optimized system of APTS contained 17% (w/w) PEG-8000 and 12.2% (w/w) potassium phosphate.

The feasible use of aqueous two-phase systems (ATPSs) to establish a viable protocol for the recovery of laccase from processed Hericium erinaceus (Bull.:Fr.) Pers. fruiting bodies was evaluated. Cold-stored (4.00 ± 1.00°C) H. erinaceus recorded the highest laccase activities of 2.02 ± 0.04 U/mL among all the processed techniques. The evaluation was carried out in twenty-five ATPSs, which composed of polyethylene glycol (PEG) with various molecular weights and potassium phosphate salt solution to purify the protein from H. erinaceus. Optimum recovery condition was observed in the ATPS which contained 17% (w/w) PEG with a molecular weight of 8000 and 12.2% (w/w) potassium phosphate solution, at a volume ratio (VR) of 1.0. The use of ATPS resulted in one-single primary recovery stage process that produced an overall yield of 99% with a purification factor of 8.03 ± 0.46. The molecular mass of laccases purified from the bottom phase was in the range of 55–66 kDa. The purity of the partitioned laccase was confirmed with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 122, Issue 3, September 2016, Pages 301–306
نویسندگان
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