کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1995927 1541479 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isoprostanes and hepatic fibrosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Isoprostanes and hepatic fibrosis
چکیده انگلیسی
After a brief introduction to oxidative stress, the discovery of F2-isoprostanes as specific and reliable markers of oxidative stress is described. Isoprostanes are also agonists of important biological effects. Since a relation between oxidative stress and collagen hyperproduction has been previously suggested and since lipid peroxidation products have been proposed as possible mediators of liver fibrosis, we investigated whether collagen synthesis is induced by F2-isoprostanes the most proximal products of lipid peroxidation. In a rat model of carbon tetrachloride-induced hepatic fibrosis, plasma isoprostanes were markedly elevated for the entire experimental period; hepatic collagen content was also increased. When hepatic stellate cells from normal liver were cultured up to activation (expression of α-smooth muscle-alpha actin) and then treated with F2-isoprostanes in the concentration range found in the in vivo studies (10−9-10−8M), a striking increase in DNA synthesis, in cell proliferation and in collagen synthesis was observed. Moreover, F2-isoprostanes increased the production of transforming growth factor-β1 by U937 cells, assumed as a model of Kupffer cells or liver macrophages. The data suggest the possibility that F2-isoprostanes generated by lipid peroxidation in hepatocytes mediate hepatic stellate cell proliferation and collagen hyperproduction seen in hepatic fibrosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Aspects of Medicine - Volume 29, Issues 1–2, February–April 2008, Pages 43-49
نویسندگان
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