کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997107 1065541 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Activation Potential of MOF Is Constrained for Dosage Compensation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The Activation Potential of MOF Is Constrained for Dosage Compensation
چکیده انگلیسی

SummaryThe H4K16 acetyltransferase MOF plays a crucial role in dosage compensation in Drosophila but has additional, global functions. We compared the molecular context and effect of MOF in male and female flies, combining chromosome-wide mapping and transcriptome studies with analyses of defined reporter loci in transgenic flies. MOF distributes dynamically between two complexes, the dosage compensation complex and a complex containing MBD-R2, a global facilitator of transcription. These different targeting principles define the distribution of MOF between the X chromosome and autosomes and at transcription units with 5′ or 3′ enrichment. The male X chromosome differs from all other chromosomes in that H4K16 acetylation levels do not correlate with transcription output. The reconstitution of this phenomenon at a model locus revealed that the activation potential of MOF is constrained in male cells in the context of the DCC to arrive at the 2-fold activation of transcription characteristic of dosage compensation.


► Dosage compensation results from strong stimulation and counteracting modulation
► The acetyltransferase MOF distributes dynamically between two distinct complexes
► The H4K16 acetylation levels correlate with transcription except for on the male X
► The MOF interactor MBD-R2 is globally involved in transcription stimulation

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 38, Issue 6, 25 June 2010, Pages 815–826
نویسندگان
, , , , ,