کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1997288 1065566 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Colocalization of Sensors Is Sufficient to Activate the DNA Damage Checkpoint in the Absence of Damage
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Colocalization of Sensors Is Sufficient to Activate the DNA Damage Checkpoint in the Absence of Damage
چکیده انگلیسی

SummaryPrevious work on the DNA damage checkpoint in Saccharomyces cerevisiae has shown that two complexes independently sense DNA lesions: the kinase Mec1-Ddc2 and the PCNA-like 9-1-1 complex. To test whether colocalization of these components is sufficient for checkpoint activation, we fused these checkpoint proteins to the LacI repressor and artificially colocalized these fusions by expressing them in cells harboring Lac operator arrays. We observed Rad53 and Rad9 phosphorylation, Sml1 degradation, and metaphase delay, demonstrating that colocalization of these sensors is sufficient to activate the checkpoint in the absence of DNA damage. Our tethering system allowed us to establish that CDK functions in the checkpoint pathway downstream of damage processing and checkpoint protein recruitment. This CDK dependence is likely, at least in part, through Rad9, since mutation of CDK consensus sites compromised its checkpoint function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 30, Issue 3, 9 May 2008, Pages 267–276
نویسندگان
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