کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1997384 | 1065575 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Shifts in Replication Timing Actively Affect Histone Acetylation during Nucleosome Reassembly Shifts in Replication Timing Actively Affect Histone Acetylation during Nucleosome Reassembly](/preview/png/1997384.png)
SummaryThe entire genome is replicated in a programmed manner, with specific regions undergoing DNA synthesis at different times in S phase. Active genes generally replicate in early S phase, while repressed genes replicate late, and for some loci this process is developmentally regulated. Using a nuclear microinjection system, we demonstrate that DNA sequences originally packaged into nucleosomes containing deacetylated histones during late S become reassembled with acetylated histones after undergoing replication in early S. Conversely, a change from early to late replication timing is accompanied by repackaging into nucleosomes containing deacetylated histones. This is carried out by differential cell-cycle-controlled acetylation and deacetylation of histones H3 and H4. These studies provide strong evidence that switches in replication timing may play a role in the regulation of nucleosome structure during development.
Journal: - Volume 34, Issue 6, 26 June 2009, Pages 767–774