کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2002213 | 1066099 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Catalase protects cardiomyocytes via its inhibition of nitric oxide synthesis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Nitric oxide (NO) has been reported to play an important role as an effector molecule in cytokine signal transduction in cardiomyocytes. A treatment of neonatal rat ventricular cardiomyocytes with interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) induces apoptosis via an NO-dependent pathway. However, cardiomyocytes were more resistant to NO-dependent cell death in the presence of catalase, while producing inducible nitric oxide synthase. This paper reports that catalase stimulates the NF-κB-binding affinity. However, the NO synthase activity is abolished by the addition of catalase, suggesting that H2O2 is involved in NO synthesis in a posttranslation state. The catalase-induced inhibition of NO was partially but significantly reversed by H4B, an important cofactor of NO synthesis. Treatment of myocytes with IL-1β, TNF-α, and IFN-γ induced a significant increase in the formation of peroxynitrite, and a pretreatment with catalase was found to quench the production of peroxynitrite. This paper shows that the catalase activity was significantly down-regulated by H4B in a concentration-dependent manner. The treatment of H4B induced reactive oxygen species (ROS) release in cardiac cell system. These results suggest that catalase interferes with NO and peroxynitrite production as well as with the related apoptosis of cardiomyocytes. This study also shows that the catalase-induced inhibition of NO release may be reversed by H4B by the release of ROS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nitric Oxide - Volume 14, Issue 3, May 2006, Pages 189-199
Journal: Nitric Oxide - Volume 14, Issue 3, May 2006, Pages 189-199
نویسندگان
Han-Jung Chae, Ki-Chan Ha, Do-Sung Kim, Gi-Seup Cheung, Yong-Geun Kwak, Hyung-Min Kim, Young-Myeong Kim, Hyun-Ock Pae, Hun-Taeg Chung, Soo-Wan Chae, Hyung-Ryong Kim,