Conditioned place preferences (CPPs) to high-caloric “snack foods” in rat strains genetically prone vs. resistant to diet-induced obesity: Resistance to naltrexone blockade

A previous study in our laboratory using Sprague–Dawley (SD) male rats showed that conditioned place preferences (CPPs) can be learned to two different high-caloric “snack foods” — one high in sugar (Froot Loops cereal: FL) vs. one high in fat (Cheetos: C), and that both preferences were mediated by endogenous opioids. Using the same CPP apparatus and procedures, two genetic sub-strains of SD rats, one selectively bred for diet-induced obesity (DIO) vs. another bred for diet resistance to obesity (DR), were used in this investigation. The experiment determined if (a) CPPs can be created in both strains using the same high-caloric “snack foods” and, (b) if CPPs existed, were they opioid dependent. Four non-deprived groups of eight male rats, half being of each strain, were given 20 min sessions to eat either FL or C in one side of a three-chamber CPP apparatus vs. chow on the opposite side over alternating days of a 20 day period. Each predetermined side had distinctly different environmental cues. Following conditioning, rats were tested during 10 min sessions to see if CPPs existed to the “snack food” trained sides. During conditioning and testing, bodyweights, intakes of foods, and activity were measured. Both FL and C generated strong CPPs that were equivalent in both strains. In contrast to our previous study in the parent strain, doses of 0, 0.50, 1.0, 2.5, and 5.0 mg/kg of the opioid antagonist, naltrexone, had no effect on blocking these CPPs. These results show that (a) DIO and DR rats can learn CPPs (i.e., “exhibit food cravings”) as well as their parent strain after periodic access to high-caloric palatable foods, but imply that (b) some physiological system other than the endogenous opioid system mediates such learning.