کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2014868 | 1541952 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Promoter regions of potato vacuolar invertase gene in response to sugars and hormones
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کلمات کلیدی
ABAIAABAPqRT-PCRGUSSREGARE6-Benzylaminopurine - 6-بنزیلامینوپورینquantitative RT-PCR - RT-PCR کمیβ-glucuronidase - β-گلوکورونیدازindole-3-acetic acid - اسید Indole-3-استیکabscisic acid - اسید آبسزیکTranscriptional regulation - تنظیم ترانزیتیgibberellic acid - جیبرلیک اسیدPotato - سیب زمینیCis-acting element - عنصر عمل CIS
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Potato vacuolar acid invertase (StvacINV1) (β-fructofuranosidase; EC 3.2.1.26) has been confirmed to play an important role in cold-induced sweetening of potato tubers. However, the transcriptional regulation mechanisms of StvacINV1 are largely unknown. In this study, the 5â²-flanking sequence of StvacINV1 was cloned and the cis-acting elements were predicted. Histochemical assay showed that the StvacINV1 promoter governed β-glucuronidase (GUS) expression in potato leaves, stems, roots and tubers. Quantitative analysis of GUS expression suggested that the activity of StvacINV1 promoter was suppressed by sucrose, glucose, fructose, and cold, while enhanced by indole-3-acetic acid (IAA), and gibberellic acid (GA3). Further deletion analysis clarified that the promoter regions from â118 to â551, â551 to â1021, and â1021 to â1521 were required for responding to sucrose/glucose, GA3, and IAA, respectively. These findings provide essential information regarding transcriptional regulation mechanisms of StvacINV1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 69, August 2013, Pages 9-16
Journal: Plant Physiology and Biochemistry - Volume 69, August 2013, Pages 9-16
نویسندگان
Yongbin Ou, Botao Song, Xun Liu, Conghua Xie, Meng Li, Yuan Lin, Huiling Zhang, Jun Liu,