Auxin: A major player in the shoot-to-root regulation of root Fe-stress physiological responses to Fe deficiency in cucumber plants

The aim of this study was to investigate the effects of IAA and ABA in the shoot-to-root regulation of the expression of the main Fe-stress physiological root responses in cucumber plants subjected to shoot Fe functional deficiency. Changes in the expression of the genes CsFRO1, CsIRT1, CsHA1 and CsHA2 (coding for Fe(III)-chelate reductase (FCR), the Fe(II) transporter and H+-ATPase, respectively) and in the enzyme activity of FCR and the acidification capacity were measured. We studied first the ability of exogenous applications of IAA and ABA to induce these Fe-stress root responses in plants grown in Fe-sufficient conditions. The results showed that IAA was able to activate these responses at the transcriptional and functional levels, whereas the results with ABA were less conclusive. Thereafter, we explored the role of IAA in plants with or without shoot Fe functional deficiency in the presence of two types of IAA inhibitors, affecting either IAA polar transport (TIBA) or IAA functionality (PCIB). The results showed that IAA is involved in the regulation at the transcriptional and functional levels of both Fe root acquisition (FCR, Fe(II) transport) and rhizosphere acidification (H+-ATPase), although through different, and probably complementary, mechanisms. These results suggest that IAA is involved in the shoot-to-root regulation of the expression of Fe-stress physiological root responses.

► The MS PLAPHI-D-10-00359R1 presents the following new points.
► Auxin role in Fe-deficiency shoot-to-root signaling.
► Auxin role confirmed with the use of IAA polar transport and action inhibitors.
► The mechanism of IAA action on H+-ATPase must involve two different auxin-dependent pathways.
► Proposed model of IAA role in the regulation of Fe-stress root responses under shoot Fe functional deficiency.