کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2015740 1541982 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
rHsp90 gene expression in response to several environmental stresses in rice (Oryza sativa L.)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
rHsp90 gene expression in response to several environmental stresses in rice (Oryza sativa L.)
چکیده انگلیسی

In this study, the gene for a rice (Oryza sativa L.) 90 kDa heat shock protein (rHsp90, GenBank accession no. AB037681) was identified by screening rice root cDNAs that were up-regulated under carbonate (NaHCO3) stress using the method of differential display, and cloned. The open-reading-frame of rHsp90-cDNA was predicted to encode a protein containing 810 amino acids, which showed high similarity to proteins in Hordeum vulgare (accession no. X67960) and Catharathus roseus (accession no. L14594). Further studies showed that rHsp90 mRNA accumulated following exposure to several abiotic stresses, including salts (NaCl, NaHCO3 and Na2CO3), desiccation (using polyethylene glycol), high pH (8.0 and 11.0) and high temperature (42 and 50 °C). Yeast (Saccharomyces cerevisiae) over-expressing rHsp90 exhibited greater tolerance to NaCl, Na2CO3 and NaHCO3 and tobacco seedlings over-expressing rHsp90 could tolerate salt concentrations as high as 200 mM NaCl, whereas untransformed control seedlings couldn't. These results suggest that rHsp90 plays an important role in multiple environmental stresses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 44, Issues 5–6, May–June 2006, Pages 380–386
نویسندگان
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