Nitrite regulates distribution of excitation energy between the two photosystems by causing state transition

The mechanism of distribution of absorbed excitation energy between the two photosystems in the presence of nitrite has been investigated in spinach (Spinacia oleracea L.) thylakoid membranes. Nitrite inhibited PS II activity (H2O → DCPIP reaction) and enhanced PS I activity (DCPIPH2 → MV reaction). Nitrite decreased the Fv/Fm ratio measured at room temperature and increased the F730/F685 ratio measured at low temperature (77 K). These results suggested that nitrite caused a decrease in the excitation energy available to PS II and transferred more energy to PS I by the mechanism of state transition. Measurement of fluorescence excitation spectra at 77 K showed that nitrite increased the absorption cross-section of PS I antenna at the expense of chlorophyll b and LHC II. Based on these observations we have suggested a role of nitrite in causing state transition.