کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016068 1541958 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Expression of a Medicago falcata small GTPase gene, MfARL1 enhanced tolerance to salt stress in Arabidopsis thaliana
چکیده انگلیسی

To understand the role of small GTPases in response to abiotic stress, we isolated a gene encoding a small GTPase, designated MfARL1, from a subtracted cDNA library in Medicago falcata, a native legume species in semi-arid grassland in northern China. The function of MfARL1 in response to salt stress was studied by expressing MfARL1 in Arabidopsis. Wild-type (WT) and transgenic plants constitutively expressing MfARL1 showed comparable phenotype when grown under control conditions. Germination of seeds expressing MfARL1 was less suppressed by salt stress than that of WT seeds. Transgenic seedlings had higher survival rate than WT seedlings under salt stress, suggesting that expression of MfARL1 confers tolerance to salt stress. The physiological and molecular mechanisms underlying these phenomena were elucidated. Salt stress led to a significant decrease in chlorophyll contents in WT plants, but not in transgenic plants. Transgenic plants accumulated less amounts of H2O2 and malondialdehyde than their WT counterparts under salt stress, which can be accounted for by the higher catalase activities, lower activities of superoxide dismutase, and peroxidase in transgenic plants than in WT plants. Transgenic plants displayed lower Na+/K+ ratio due to less accumulation of Na+ than wild-type under salt stress conditions. The lower Na+/K+ ratio may result from less accumulation of Na+ due to reduced expression of AtHKT1 that encodes Na+ transporter in transgenic plants under salt stress. These findings demonstrate that MfARL1 encodes a novel stress-responsive small GTPase that is involved in tolerance to salt stress.


► A small GTPase gene, MfARL1 isolated from M. falcata, was expressed in Arabidopsis.
► Transgenic plants accumulated less H2O2 and malondialdehyde under salt stress.
► Transgenic plants showed reduced Na+ content due to suppression of AtHKT1 expression.
► MfARL1 enhanced tolerance of the transgenic Arabidopsis to salt stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 63, February 2013, Pages 227–235
نویسندگان
, , , , ,