Brassica juncea nitric oxide synthase like activity is stimulated by PKC activators and calcium suggesting modulation by PKC-like kinase

► Nitric oxide synthase like activity (BjNOSla) was detected in B. juncea seedlings. ► BjNOSla is activated by calcium and NADPH and is inhibited by NBT and l-NAME. ► NADPH-diaphorase and DAF-2DA staining showed its localization in guard cells. ► BjNOSla was enhanced by PMA, PS with 1,2-DG6, light and abiotic stress. ► Inhibition by staurosporine and calphostin, indicate it to be PKC regulated.