کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
20162 | 43160 | 2015 | 6 صفحه PDF | دانلود رایگان |
Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σB- and σA-dependent promoters constitutively improved the yield of recombinant protein approximately 2–3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σB-promoter was located upstream to σA-promoter but independent to its orientation. A conserved element in either −10 or −35 box of σB-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.
Journal: Journal of Bioscience and Bioengineering - Volume 120, Issue 4, October 2015, Pages 470–475