Expression of arabinogalactan proteins during tomato fruit ripening and in response to mechanical wounding, hypoxia and anoxia

Arabinogalactan proteins (AGPs) are highly glycosylated members of the superfamily of hydroxyproline-rich glycoproteins (HRGPs). Despite their implication in many aspects of plant growth and development little is known about their role in tomato fruit ripening (Solanum lycopersicum) and their response to abiotic stress in tomato fruits. A search of the currently available tomato genome database resulted in the identification of 34 genes encoding putative AGPs, with at least 20 of them being expressed in fruit. We monitored the abundance of AGPs bound by JIM8 and JIM13 monoclonal antibodies as well as the gene expression profiles of the Lys-rich LeAGP1 and two classical AGPs, SlAGP2 and SlAGP4. The JIM8- and JIM13-bound AGPs showed constitutive expression during fruit ripening and under hypoxic conditions, slight up-regulation to mechanical wounding in excised tomato fruit pericarp discs and up-regulation under anoxia indicating functional roles for these proteins in the developmental program of ripening and in response to abiotic stresses. Moreover, the SlAGP2 mRNA was significantly up-regulated during fruit ripening following the climacteric ethylene production, a pattern of expression similar to that of tomato fruit PG. The SlAGP4 and LeAGP1 mRNAs were up-regulated in response to mechanical wounding while under anoxia only the SlAGP4 transcript was induced. The protein and mRNA levels of these AGPs were induced under mechanical wounding while only JIM8-bound AGPs and SIAGP4 expression were induced under anoxic conditions. Our results indicate that selected tomato AGPs seem to play a role in fruit ripening as well as in response to mechanical wounding and anoxia.

► We identified 34 genes encoding putative AGPs, 20 of them being expressed in fruit.
► JIM8- and JIM13-bound AGPs were constitutively expressed during fruit ripening and under hypoxia.
► JIM8- and JIM13-bound AGPs were up-regulated in response to mechanical wounding and anoxia.
► SlAGP2 mRNA is induced during fruit ripening.
► Mechanical wounding and anoxia up-regulated SlAGP4 mRNA.