Kinetic study of sunflower phospholipase Dα: Interactions with micellar substrate, detergents and metals

► Purified sunflower phospholipase Dα was inactive in vitro on bilamellar substrates. ► PLD was active on mixed micelles made with phospholipids and Triton-X100/SDS mixture. ► This enzyme was able to bind calcium with a dissociation constant of 40-50 mM.