Molecular cloning, characterization, and expression of an alfalfa (Medicago sativa L.) heme oxygenase-1 gene, MsHO1, which is pro-oxidants-regulated

► MsHO1 encodes a HO-1 protein. ► The MsHO1:GFP fusion protein was localized in the chloroplast. ► The biochemical activity of mature MsHO1 expressed in Escherichia coli was determined. ► MsHO1 was induced significantly by some pro-oxidant compounds.