کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016480 | 1067663 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression analysis of cinnamoyl-CoA reductase (CCR) gene in developing seedlings of Leucaena leucocephala: A pulp yielding tree species
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کلمات کلیدی
BCIPLeucaena leucocephalaPoly Acrylamide Gel Electrophoresiscinnamoyl-CoA reductasenitro-blue tetrazolium chlorideAMV-RTPAGENBTDFRqRT-PCRSyringyl unitkDaCCRcDNA - cDNAComplementary DNA - DNA تکمیلیEnzyme-Linked Immuno-Sorbent Assay - آنزیم مرتبط با ایمونوسیبنیتهLignin biosynthesis - بیوسنتز لیگنینELISA - تست الیزاrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدRace - مسابقهquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیavian myeloblastosis virus reverse transcriptase - ویروس معروف ویرولواستاز ویروس معدهkilo Daltons - کیلوگرم دالتون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Removal of lignin is a major hurdle for obtaining good quality pulp. Leucaena leucocephala (subabul) is extensively used in paper industry in India; therefore, as a first step to generate transgenic plants with low lignin content, cDNA and genomic clones of CCR gene were isolated and characterized. The cDNA encoding CCR (EC 1.2.1.44) was designated as Ll-CCR; the sequence analysis revealed an Open Reading Frame (ORF) of 1005Â bp. Phylogenetic analysis showed that Ll-CCR sequence is highly homologous to CCRs from other dicot plants. The 2992Â bp genomic clone of Leucaena CCR consists of 5 exons and 4 introns. The haploid genome of L. leucocephala contains two copies as revealed by DNA blot hybridization. Ll-CCR gene was over-expressed in Escherichia coli, which showed a molecular mass of approximately 38Â kDa. Protein blot analysis revealed that Ll-CCR protein is expressed at higher levels in root and in stem, but undetectable in leaf tissues. Expression of CCR gene in Leucaena increased up to 15Â d in case of roots and stem as revealed by QRT-PCR studies in 0-15Â d old seedlings. ELISA based studies of extractable CCR protein corroborated with QRT-PCR data. CCR protein was immuno-cytolocalized around xylem tissue. Lignin estimation and expression studies of 5, 10 and 15Â d old stem and root suggest that CCR expression correlates with quantity of lignin produced, which makes it a good target for antisense down regulation for producing designer species for paper industry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 49, Issue 2, February 2011, Pages 138-145
Journal: Plant Physiology and Biochemistry - Volume 49, Issue 2, February 2011, Pages 138-145
نویسندگان
Sameer Srivastava, Ranadheer K. Gupta, Manish Arha, Rishi K. Vishwakarma, Shuban K. Rawal, P.B. Kavi Kishor, Bashir M. Khan,