کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016635 1541980 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
cDNA microarray analysis of gene expression in Brassica napus treated with oligochitosan elicitor
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
cDNA microarray analysis of gene expression in Brassica napus treated with oligochitosan elicitor
چکیده انگلیسی

An oilseed rape (Brassica napus H) cDNA microarray containing 8095 expressed sequence tags (ESTs) was used to analyze the B. napus gene expression changes elicited by oligochitosan. Transcript levels for 393 genes were altered twofold or more in oligochitosan-treated seedlings compared to control seedlings. Of the 393 genes, 257 were repressed and 136 were induced. Semi quantification RT-PCR of eight of these 393 genes confirmed the microarray results. These 393 genes were involved in different processes and had different functions including defense, primary metabolism, transcription, and signal transduction etc. Some of these genes were elicited by various pathogen-related or stress stimuli, and others were regulated by plant growth regulators like auxin and gibberellin. These manifested complicated interactions of oligochitosan and phytohormones. An important jasmonic acid (JA) synthase (2-oxophytodienoate-10,11-reductase) gene, a JA-mediated defense required kinase ATMPK4-homolog gene, an ethylene receptor gene, and two ethylene responsive element binding protein (EREBP) genes were induced by oligochitosan, suggesting that oligochitosan activated the plant self-defense through JA/ET signaling pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 44, Issues 11–12, November–December 2006, Pages 910–916
نویسندگان
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