کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016754 | 1541981 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification, product characterization and kinetic properties of lipoxygenase from olive fruit (Olea europaea L.)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purification, product characterization and kinetic properties of lipoxygenase from olive fruit (Olea europaea L.) Purification, product characterization and kinetic properties of lipoxygenase from olive fruit (Olea europaea L.)](/preview/png/2016754.png)
چکیده انگلیسی
Lipoxygenase from olive fruit was purified to homogeneity for the first time after differential centrifugations and by hydrophobic chromatography. The enzyme had a molecular mass of 98 kDa and exhibited a maximal activity at pH 6. Lipoxygenase had a better affinity for linoleic acid (Km = 82.44 μM) than for linolenic acid (Km = 306.26 μM). It is inhibited by linoleate:oxygen oxidoreductase (LOX) inhibitors like nordihydroguaiaretic acid (NDGA) or propyl gallate. The reaction product was 13-hydroperoxy octadecadienoic acid when linoleic acid was used as substrate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 44, Issues 7–9, July–September 2006, Pages 450–454
Journal: Plant Physiology and Biochemistry - Volume 44, Issues 7–9, July–September 2006, Pages 450–454
نویسندگان
V. Lorenzi, J. Maury, J. Casanova, L. Berti,