کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016854 1067702 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses
چکیده انگلیسی

In sunflower, asparagine synthetase (AS; EC 6.3.5.4) is encoded by a small family of three genes (HAS1, HAS1.1 and HAS2) that are differentially regulated by light, carbon and nitrogen availability. In this study, the response of each gene to various stress conditions was examined by Northern analysis with gene-specific probes in leaves and roots. The expression of HAS1 and HAS1.1 genes was induced by osmotic stress (300 mM mannitol), salt stress (150 mM NaCl), and heavy-metal stress (20 μM CuSO4), more in roots than in leaves. The expression of HAS2 was not significantly altered by stress treatments. The positive response of HAS1 and HAS1.1 genes to osmotic and salt stresses occurred in the light, in contrast to that previously found in unstressed plants. Measurements of sucrose and total free amino acid contents in leaves and roots indicate that the expression of root HAS1 and HAS1.1 genes in stressed plants is not under metabolic control by the intracellular C/N ratio, suggesting the involvement of some specific stress factor(s). Growth of plants at 40 °C for 12 h negatively affected the expression of HAS1 and HAS1.1 but not that of HAS2.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 45, Issue 1, January 2007, Pages 33–38
نویسندگان
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