Evaluation of abiotic stress tolerance in transgenic potato plants with reduced expression of PSII manganese stabilizing protein

Manganese stabilizing protein (MSP) is an important component of the Photosystem II (PSII) oxygen evolving complex. In our previous work, transgenic potato plants with reduced expression of MSP (MSP-As) were developed and their physiological and biochemical responses were studied. In this report, we address the response of MSP-As plants toward salinity, heavy metal and osmotic stresses. MSP-As plants treated with NaCl, ZnCl2 or mannitol solution showed significant level of tolerance under all the stress conditions. Specific enzyme activities of major ROS-scavenging enzymes were found significantly higher in MSP-As plants than the control plants. MSP-As plants accumulated increased levels of proline and low molecular weight metabolites such as ascorbate and α-tocopherol, which indicated that these plants were much more resistant to stress compared to the corresponding control plants. The primary photochemical efficiencies and the OJIP kinetics analyses further confirmed that MSP-As plants were in better optimal health under stress compared to the control plants. Although the exact reason behind the increased stress tolerance in stressed MSP-As plants is unclear, our results strongly indicate the role of MSP of unknown function in abiotic stress tolerance.

► Downregulated MSP in potato results in lack of intact oxygen evolving complex.
► Antisense MSP plants showed resistance against salinity, heavy metal and osmotic stress along with increased tuber yield.
► Downregulated MSP caused increased sugars, ascorbate, tocopherol, ABA and proline accumulation.
► Chl a transient kinetics confirms better optimal health of MSP-As plants under stress.