کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2017500 | 1067800 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant](/preview/png/2017500.png)
The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2 cM region on chromosome 5.
► The reduced levels of CysP prolamin bands are a characteristic of the esp1 mutation.
► The prolamins derived from multiple genes were affected by the esp1 mutation.
► Esp1 locus encodes a regulatory factor involved in CysP prolamin molecules.
► The structural genes for bands reduced in esp1 mutant formed a gene cluster.
Journal: Plant Science - Volume 181, Issue 2, August 2011, Pages 125–131