AtNG1 encodes a protein that is required for seed germination

The pentatricopeptide repeat (PPR) family of eukaryotic proteins has numerous members in plants and is important for plant development. In the present study, we cloned a novel PPR gene, designated AtNG1, and characterized the ng1 Arabidopsis mutant. Morphological and structural observation of an ng1 mutant revealed that its sexual reproduction and seed formation processes are essentially normal. The mature embryonic root of ng1 is fully developed and has a well-differentiated structure; however, ng1 seeds cannot germinate, even when supplied with supplemental hormones and nutrition. Further investigation showed that embryo expansion and root cell elongation fails to occur after water imbibitions. Transient gene expression analysis indicated that AtNG1 localizes in mitochondrion. This implies that the deficiency of mitochondrion function might be the reason for the failed seed germination. Thus, our finding confirmed that AtNG1 plays a critical role in the early process of seed germination.

► AtNG1 encodes a typical PPR protein.
► Mature embryo of ng1 mutant is fully developed and well differentiated.
► Embryo expansion and root cell elongation fail to occur during ng1 seed germination.
► ng1 appears to prevent activation of the seed germination process, indicating a previously unknown function of the PPR protein family.