Light qualities and dose influence ascorbate pool size in detached oat leaves

In this work, we studied the mechanism of light influence on AsA pool size in Avena sativa L. under the effects of low intensity light at different wavelengths. Exposure to low intensity light of oat leaf segments incubated in water or in l-galactono-1,4-lactone (GL), resulted in an increase in AsA content compared with the dark control. This increase was due to modulation of l-galactono-1,4-lactone dehydrogenase (GLDH; EC 1.3.2.3) light-dependent activity and was dependent on the size of the endogenous GL pool. Both blue and red light were effective in increasing AsA, and this increase depended on both exposure time and light intensity. Protein biosynthesis, photosynthesis and calcium were involved in controlling the level of light-dependent AsA. We suggest that multiple checkpoints correlated to the presence of light underlie the ascorbate pool size. The presence of a light-activated switch for the maintenance of an adequate AsA level seems to be necessary for the various tasks of scavenging reactive oxygen species, in response to the dark-light cycle which plants experience under natural conditions.

► The low light may modulate the level of l-galactono-1,4-lactone dehydrogenase (GLDH).
► The GLDH activity depends on endogenous l-galactono-1,4-lactone pool.
► A light-activated switch maintains an appropriate ascorbate level for ROS removal.
► Multiple checkpoints correlated to the light underlie the ascorbate pool size in oat.