کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2017949 | 1067826 | 2009 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Proteomic pattern-based identification of molecular phenotypes from Arabidopsis thaliana ecotypes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Ecotypes are geographic variants of plants. Using two-dimensional gel electrophoresis, we performed a comparative analysis of intra-species proteome variation in Arabidopsis ecotypes. The effect of single gene mutations on parental ecotype proteome patterns was also examined. Three Arabidopsis ecotypes, Col-0, Ler-0 and Ws-0, were compared to identify characteristic proteome patterns. From the protein expression profile of whole seedling protein extracts, 13 differentially expressed group of proteins were distinguished by self-organizing map clustering analysis. Proteins in each cluster were identified by peptide mass fingerprinting. These isolated clusters were used as a molecular phenotype for comparison of Col-0, Ler-0, and Ws-0 with genetically close or distant ecotypes, as well as several single locus mutants. Comparison of protein patterns with other ecotypes showed that the genetic diversity of the ecotypes was reflected in quantitative differences in the protein expression patterns. Single locus mutations usually did not alter the parental protein pattern significantly, although a few mutations did cause a dramatic change relative to the parental pattern.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 177, Issue 1, July 2009, Pages 7-18
Journal: Plant Science - Volume 177, Issue 1, July 2009, Pages 7-18
نویسندگان
Kyung Mok Park, Yoon Hee Choi, Won Man Park, Hyung Jin Na, Yun Jeong Na, Dong Hee Lee, Won Il Chung, Dong Su Kim,