کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2018059 | 1067832 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microarray analysis of 7029 gene expression patterns in burma mangrove under high-salinity stress
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To identify genes of potential importance to salt tolerance in burma mangrove (Bruguiera gymnorrhiza), we analyzed the gene expression profiles in salt-stressed burma mangrove using cDNA microarray containing 7029 clones based on 14,842 expressed sequence tags (ESTs). Combined results from all hybridization, we identified 287 genes with a greater than 5-fold change at least 1 time point after salt treatment (500Â mM) compared with control water-treated plant; 228 genes were up-regulated more than five-fold and 61 genes were suppressed less than one-fifth. Venn diagram analysis showed tissue-specific and -overlapping of these highly changed genes for up- and down-regulation, respectively. These highly up- and down- regulated genes were characterized using gene ontology. Based on these genes, we conducted a hierarchical clustering analysis to reveal the patterns of gene expression. The analysis identified 6 major co-expression clusters that support the interpretation of an adaptive process to high-salinity environment. The microarray results were validated by real-time quantitative PCR on 3 selected genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 172, Issue 5, May 2007, Pages 948-957
Journal: Plant Science - Volume 172, Issue 5, May 2007, Pages 948-957
نویسندگان
Masashi Miyama, Nobutaka Hanagata,