کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2019554 1542211 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of acute and chronic DSS induced colitis on plasma eicosanoid and oxylipin levels in the rat
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effect of acute and chronic DSS induced colitis on plasma eicosanoid and oxylipin levels in the rat
چکیده انگلیسی


• Chronical DSS treatment of rats resulted in increased colon prostaglandin levels.
• Plasma oxylipin levels are unchanged during chronic phase of colitis.
• Plasma hydroxy, epoxy and dihydroxy fatty acids elevated in acute phase of colitis.

Eicosanoids and oxylipins are potent lipid mediators involved in the regulation of inflammation. In order to evaluate their role and suitability as biomarkers in colitis, we analyzed their systemic levels in the acute and chronic phase of dextran sulfate sodium (DSS) induced colitis. Male Fischer 344 rats were treated in three cycles with 4% DSS in the drinking water (4 days followed by 10 days recovery) and blood was drawn 3 days prior to the first DSS treatment and on days 4, 11, 32 and 39. Histopathological evaluation of the colon tissue after 42 days showed that the animals developed a mild to severe chronic colitis. Consistently, prostaglandin levels were massively (twofold) elevated in the colonic tissue. LC–MS based targeted metabolomics was used to determine plasma oxylipin levels at the different time points. In the acute phase of inflammation directly after DSS treatment, epoxy-fatty acid (FA), dihydroxy-FA and hydroxy-FA plasma concentrations were uniformly elevated. With each treatment cycle the increase in these oxylipin levels was more pronounced. Our data suggest that in the acute phase of colitis release of polyunsaturated FAs from membranes in the inflamed tissue is reflected by a uniform increase of oylipins formed in different branches of the arachidonic acid cascade. However, during the recovery phases the systemic oxylipin pattern is not or only moderately altered and does not allow to evaluate the onset of chronic inflammation in the colon.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins & Other Lipid Mediators - Volume 120, July 2015, Pages 155–160
نویسندگان
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