کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020235 1542318 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain
چکیده انگلیسی


• Optimized expression system and purification of recombinant Lac repressor is described.
• Biophysical quantities of isotopically enriched protein for NMR are accessible.
• Lac repressor regulatory domain can be expressed without toxicity in high yield.
• Solution NMR confirms Lac proteins are highly homogeneous and functional.

The recombinant production of Lac repressor (LacI) in Escherichia coli is complicated by its ubiquitous use as a regulatory element in commercially-available expression vectors and host strains. While LacI-regulated expression systems are often used to produce recombinant LacI, the product can be heterogeneous and unsuitable for some studies. Alternative approaches include using unregulated vectors which typically suffer from low yield or vectors with promoters induced by metabolically active sugars which can dilute isotope labels necessary for certain biophysical studies. Here, an optimized expression system and isolation protocol for producing various constructs of LacI is introduced which eliminates these complications. The expression vector is an adaptation of the pASK backbone wherein expression of the lacI gene is regulated by an anhydrotetracyline inducible tetA promoter and the host strain lacks the lacI gene. Typical yields in highly deuterated minimal medium are nearly 2-fold greater than those previously reported. Notably, the new expression system is also able to produce the isolated regulatory domain of LacI without co-expression of the full-length protein and without any defects in cell viability, eliminating the inconvenient requirement for strict monitoring of cell densities during pre-culturing. Typical yields in highly deuterated minimal medium are significantly greater than those previously reported. Characterization by solution NMR shows that LacI constructs produced using this expression system are highly homogenous and functionally active.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 123, July 2016, Pages 75–82
نویسندگان
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