کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2020533 1069186 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioseparation of papain from Carica papaya latex by precipitation of papain–poly (vinyl sulfonate) complexes
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Bioseparation of papain from Carica papaya latex by precipitation of papain–poly (vinyl sulfonate) complexes
چکیده انگلیسی


• Papain is an endopeptidase used in many industrial processes.
• Usually, papain is isolated from papaya latex by different precipitation methods.
• We propose a novel technique based on precipitation of papain–PVS complexes.
• The recovery is around 87% and the purification factor is 2.

The formation of insoluble complexes between enzymes and polyelectrolytes is a suitable technique for isolating these biomolecules from natural sources, because it is a simple and rapid technique that allows the concentration of the protein. This technique can be used in most purification protocols at the beginning of the downstream process.The aim of this investigation is to isolate papain from Carica papaya latex by precipitation of insoluble complexes between this enzyme and poly (vinyl sulfonate).The papain–poly (vinyl sulfonate) complex was insoluble at pH lower than 6, with a PVS/PAP stoichiometric ratio of 1:279. Ionic strength affected the complex formation. The presence of the polymer increased the enzymatic activity and protected the enzyme from autodegradation. The optimal conditions for the formation of insoluble papain–polyelectrolyte complex formation were applied to C. papaya latex and a high recovery was obtained (around 86%) and a purification factor around 2. This method can be applied as an isolation method of papain from C. papaya latex or as a first step in a larger purification strategy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 91, Issue 1, September 2013, Pages 91–95
نویسندگان
, , , , , ,