کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2020559 | 1069188 | 2013 | 9 صفحه PDF | دانلود رایگان |
Expression of recombinant proteins in bacterial or eukaryotic systems often results in aggregation rendering them unavailable for biochemical or structural studies. Protein aggregation is a costly problem for biomedical research. It forces research laboratories and the biomedical industry to search for alternative, more soluble, non-human proteins and limits the number of potential “druggable” targets. In this study we present a highly reproducible protocol that introduces the systematic use of an extensive number of detergents to solubilize aggregated proteins expressed in bacterial and eukaryotic systems. We validate the usefulness of this protocol by solubilizing traditionally difficult human protein targets to milligram quantities and confirm their biological activity. We use this method to solubilize monomeric or multimeric components of multi-protein complexes and demonstrate its efficacy to reconstitute large cellular machines. This protocol works equally well on cytosolic, nuclear and membrane proteins and can be easily adapted to a high throughput format.
► Efficient, high-throughput method to solubilize aggregated and insoluble proteins.
► Successfully tested in bacterial and eukaryotic expression systems.
► Successfully used for membrane, protoplasmic and multi-protein complexes.
► Protein yields in milligram quantities allow biochemical and industrial applications.
Journal: Protein Expression and Purification - Volume 87, Issue 2, February 2013, Pages 111–119