Purification and characterization of the recombinant human prostaglandin H synthase-2 expressed in Pichia pastoris

► Expression of functional human PGHS-2 in Pichia pastoris. ► Characterization of human recombinant PGHS-2 by kinetic constants Km, kcat and IC50. ► Purification of C-terminally His6-tagged human PGHS-2.