Large-scale production of soluble recombinant amyloid-β peptide 1-42 using cold-inducible expression system

► Expression of Aβ1-42 using pCold vector system. ► Increased expression and solubility of Aβ1-42 at sub-optimal growth temperature. ► A simple purification using affinity chromatography without denaturation. ► Recombinant Aβ1-42 induces cytotoxicity on SH-SY5Y cells.