کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021127 1069229 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning, expression, and characterization of a new deoxyribose 5-phosphate aldolase from Yersinia sp. EA015
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cloning, expression, and characterization of a new deoxyribose 5-phosphate aldolase from Yersinia sp. EA015
چکیده انگلیسی

A new deoC gene encoding deoxyribose 5-phosphate aldolase (DERA) was identified in Yersinia sp. EA015 isolated from soil. The DERA gene had an open reading frame (ORF) of 672 base pairs encoding 223 amino acids to yield a protein of molecular mass 24.8 kDa. The amino acid sequence was 94% identical to that of DERA from Yersinia intermedia ATCC 29909. DERA was over-expressed in Escherichia coli and purified using Ni–NTA affinity chromatography. The specific activity was 137 μmol/min/mg. The Michaelis constant (km value) of DERA was 9.1 mM. DERA was optimally active at pH 6.0 and 50 °C. DERA was tolerant to a high concentration (300 mM) of acetaldehyde.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 68, Issue 2, December 2009, Pages 196–200
نویسندگان
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