کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021281 1069238 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single-step production of functional OEP24 proteoliposomes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Single-step production of functional OEP24 proteoliposomes
چکیده انگلیسی
The pea chloroplastic outer envelope protein OEP24 is a voltage-dependent channel that can function as a general solute channel in plants. OEP24 is a close functional homologue of VDAC which, in mammalian cells, modulates the permeability of the outer mitochondrial membrane. Here, we describe the production in a one-step reaction of active OEP24 in proteoliposomes or in soluble form using a cell-free expression system. We combine evidence from electrophysiological experiments, biophysical characterization, and biochemical analysis demonstrating that OEP24 is present as a functional channel in liposomes. Thus, production of OEP-containing proteoliposomes may provide a helpful tool for deciphering the role of the OEP family members.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 69, Issue 1, January 2010, Pages 106-111
نویسندگان
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