کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021597 | 1069253 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An E. coli expression system optimized for DELLA proteins
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An E. coli expression system optimized for DELLA proteins An E. coli expression system optimized for DELLA proteins](/preview/png/2021597.png)
چکیده انگلیسی
The DELLA proteins are involved in regulation of plant growth in response to phytohormonal signals such as GA, ethylene, and auxin. They have become one of most challenging and active area of research due to their fundamental roles in plant biology. Here, we describe the first successful expression of the N-terminal domains of DELLA proteins of Arabidopsis thaliana and Malus domestica in Escherichia coli system which will be used to produce monoclonal antibodies for profiling protein micro-arrays. Optimizations of the cloning, expression, and purification using specific tags have been discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 58, Issue 1, March 2008, Pages 168–174
Journal: Protein Expression and Purification - Volume 58, Issue 1, March 2008, Pages 168–174
نویسندگان
Xiaolin Sun, Nicky Frearson, Christopher Kirk, William T. Jones, Dawn Harvey, Jasna Rakonjac, Toshi Foster, Taha Al-Samarrai,