کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2021753 | 1069261 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heterologous expression and in vitro assembly of the transmembrane cytochrome b6
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Heterologous expression and in vitro assembly of the transmembrane cytochrome b6 Heterologous expression and in vitro assembly of the transmembrane cytochrome b6](/preview/png/2021753.png)
چکیده انگلیسی
Folding and assembly studies with α-helical membrane proteins are often hampered by the absence of high-level expression systems as well as by missing suitable in vitro refolding procedures. Experimental constraints and requirements for heterologous expression and in vitro assembly of cytochrome b6 have been examined and conditions for in vitro reconstitutions of the protein have been optimized. Cytochrome b6 can serve as an excellent model system for in vitro studies on the dynamic interplay of an apo-protein and heme cofactors during assembly of a transmembrane b-type cytochrome. In vitro assembled cytochrome b6 binds two hemes with different midpoint potentials and both ferri as well as ferro heme bind to the apo-cytochrome. However, the ferro cytochrome appears to be less stable than the ferri form.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 56, Issue 2, December 2007, Pages 279-285
Journal: Protein Expression and Purification - Volume 56, Issue 2, December 2007, Pages 279-285
نویسندگان
Alexander Prodöhl, Carolin Dreher, Ruth Hielscher, Petra Hellwig, Dirk Schneider,