کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021948 1069271 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major
چکیده انگلیسی

Leishmaniasis is considered by the World Health Organization to be the second most important disease caused by a protozoan parasite. Biochemical and molecular biology studies can help in the understanding of the biology of the Leishmania parasite. All protozoan parasites, including Leishmania, are unable to synthesize purines de novo, and nucleoside diphosphate kinases (NDK) are involved in the salvage pathway by which free purines are converted to nucleosides and subsequently to nucleotides. In this report, we describe the cloning of NDK coding-sequence from Leishmania major, the expression of the enzyme containing a His6-tag in Escherichia coli, and purification of the catalytically active native protein by affinity chromatography using Ni–NTA resin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 49, Issue 2, October 2006, Pages 244–250
نویسندگان
, , , , , ,