کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2022059 1069277 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cloning, expression, purification, and characterization of zebrafish cytosolic serine hydroxymethyltransferase
چکیده انگلیسی

A cDNA which encodes for zebrafish serine hydroxymethyltransferase (SHMT) has been cloned into a pET43.1a vector as a NdeI–EcoRI insert and transformed into HMS174(DE3) cells. After induction with isopropyl thiogalactoside, the enzyme was purified with a three-step purification protocol and about 15 mg of pure enzyme was obtained per liter of culture. Spectral and structural characteristics of the recombinant zebrafish SHMT are similar to the rabbit and human cytosolic SHMT. Kinetic constants for the natural substrates l-serine and tetrahydrofolate are also comparable to the values obtained previously for the rabbit and human cytosolic enzyme.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 46, Issue 2, April 2006, Pages 212–220
نویسندگان
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