کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022071 | 1069277 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens A rapid method for the purification of methanol dehydrogenase from Methylobacterium extorquens](/preview/png/2022071.png)
چکیده انگلیسی
Methanol dehydrogenase (MDH) is a water soluble quinoprotein that catalyzes the oxidation of methanol as an important carbon source in methylotrophic bacteria. A rapid method for the purification of MDH from Methylobacterium extorquens AM1 was developed using a single cation exchange chromatographic step, followed by ultrafiltration for final purification, enzyme concentration, and buffer exchange. MDH was obtained in an excellent overall yield with a final enzyme purity of greater than 97%. Storage at −80 °C in 20 mM phosphate buffer, pH 7.0, showed only a negligible loss of enzyme activity after six months.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 46, Issue 2, April 2006, Pages 316–320
Journal: Protein Expression and Purification - Volume 46, Issue 2, April 2006, Pages 316–320
نویسندگان
Qinfeng Liu, Jon R. Kirchhoff, Christopher R. Faehnle, Ronald E. Viola, Richard A. Hudson,