کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022106 | 1069278 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preparation of hepatitis C virus structural and non-structural protein fragments and studies of their immunogenicity Preparation of hepatitis C virus structural and non-structural protein fragments and studies of their immunogenicity](/preview/png/2022106.png)
Plasmids pQE-60 and pQE-30 containing 6× His-tag sequence were used for expression of fragments of HCV structural and non-structural proteins in Escherichia coli (E. coli). The following fragments were used: core (1–98 aa), NS3 (202–482 aa), and tetramer of hypervariable region 1 (HVR1) of E2 protein. The constructed plasmids directed high levels of expression of HCV proteins in E. coli JM109. After purification by the metal-affinity chromatography on nickel–nitrilotriacetic acid (Ni–NTA) agarose, the His-tagged HCV proteins were used for immunization of BALB/c mice. All three proteins were able to induce high levels of specific antibodies and, in the case of the NS3 and HVR1 tetramer, also to mount vigorous cell-proliferating responses. High immunogenicity of the tested fragments of HCV proteins shows them as good candidates for inclusion into the future HCV vaccine preparations.
Journal: Protein Expression and Purification - Volume 50, Issue 1, November 2006, Pages 43–48