کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2022616 1542419 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Corticotropin releasing factor—Distribution in rat intestine and role in neuroprotection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Corticotropin releasing factor—Distribution in rat intestine and role in neuroprotection
چکیده انگلیسی

Aims of the present study were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling.Networks of CRF-containing nerve cell bodies and fibers were detected in rat intestine. CRF-IR neurons contained to a high degree also VIP. A low number of cultured myenteric neurons was CRF-IR. CRF, Ucn1 or CRF-antagonist did not promote neuronal survival of cultured myenteric neurons, while VIP significantly enhanced neuronal survival. Simultaneous presence of CRF attenuated the VIP mediated increase in neuronal survival. Co-culturing neurons and mast cells resulted in a marked reduction in neuronal survival, not executed via CRF signaling pathways. Conclusion: CRF is present in enteric neurons and counteracts the neuroprotective effect of VIP in vitro.

Research Highlights
► CRF is found in enteric neurons, together with VIP, in rat intestine.
► Primary culturing of rat myenteric neurons reduces CRF expression.
► CRF counteracts VIP-induced neuroprotection of myenteric neurons.
► Mast cell-induced reduction of neuronal survival is not executed via CRF signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 166, Issues 1–3, 17 January 2011, Pages 68–75
نویسندگان
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