کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022811 | 1542421 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Novel localization of orexin A in the tubular cytotypes of the rat testis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Novel localization of orexin A in the tubular cytotypes of the rat testis Novel localization of orexin A in the tubular cytotypes of the rat testis](/preview/png/2022811.png)
چکیده انگلیسی
The hypothalamic peptides orexin A (OXA) and orexin B (OXB), deriving from the proteolytic cleavage of the precursor molecule prepro-orexin, have also been localized in multiple cerebral areas and peripheral organs. They regulate food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual behavior, arousal, and the hypothalamic/hypophyseal axes. Prepro-orexin mRNA expression and OXA-immunoreactivity were previously detected in the rat testis at different ages of postnatal development, with strong peptide signal in Leydig cells and spermatocytes. In this study, OXA-immunoreactivity was found in Sertoli cells and spermatids of rat testis. Hematoxylin-counterstained sections revealed OXA positive spermatids in the stages of the germinal epithelium cycle ranging from the VIIth to the XIVth. The expression of prepro-orexin mRNA and of the protein in the testis tissue was ascertained by reverse-transcription polymerase chain reaction and Western blotting analysis, respectively. Although the functional role of OXA in the male genital tract still remains to be elucidated, our findings provide the first evidence that Sertoli cells, belonging to the tubular compartment of testis, represent an important source of OXA, thus suggesting the potential involvement of the peptide in the control of seminiferous epithelium development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 164, Issues 2â3, 24 September 2010, Pages 53-57
Journal: Regulatory Peptides - Volume 164, Issues 2â3, 24 September 2010, Pages 53-57
نویسندگان
Simona Tafuri, Roberta Lo Muto, Luigi Michele Pavone, Salvatore Valiante, Anna Costagliola, Norma Staiano, Alfredo Vittoria,