کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022838 | 1542424 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
l-carnitine attenuates oxidant injury in HK-2 cells via ROS-mitochondria pathway
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: l-carnitine attenuates oxidant injury in HK-2 cells via ROS-mitochondria pathway l-carnitine attenuates oxidant injury in HK-2 cells via ROS-mitochondria pathway](/preview/png/2022838.png)
چکیده انگلیسی
Oxidative stress has been considered as the possible mechanism of renal ischemia/reperfusion injury. l-carnitine is an endogenous mitochondrial membrane compound and could effectively protect ischemia-reperfusion injury in the kidney. To elucidate the nephroprotective effects of l-carnitine, here we assessed the effect of l-carnitine on hydrogen peroxide (H2O2)-mediated oxidative stress in the human proximal tubule epithelial cell line, HK-2 cells. The results showed that pretreatment with l-carnitine 12Â h inhibited H2O2-induced cell viability loss, intracellular reactive oxygen species generation and lipid peroxidation in a concentration-dependent manner. Also l-carnitine promoted endogenous antioxidant defense components including total antioxidative capacity, glutathione peroxidase, catalase and superoxide dismutase. In parallel, cell apoptosis triggered by H2O2 characterized with the DNA fragment and caspase-3 activity were also inhibited by l-carnitine. Furthermore, mitochondrial dysfunction associated with cell apoptosis including membrane potential loss, down-regulation of Bcl-2 and up-regulation of Bax and the release of cytochrome c were abrogated in the presence of l-carnitine. These results suggested that l-carnitine could protect HK-2 cells from H2O2-induced injury through the inhibition of oxidative damage, mitochondria dysfunction and ultimately inhibition of cell apoptosis, which indicates that l-carnitine may be a promising approach for the treatment of oxidative stress in renal diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Peptides - Volume 161, Issues 1â3, 9 April 2010, Pages 58-66
Journal: Regulatory Peptides - Volume 161, Issues 1â3, 9 April 2010, Pages 58-66
نویسندگان
Junsheng Ye, Juan Li, Yuming Yu, Qiang Wei, Wenfeng Deng, Lixin Yu,