Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay

• Rapid and high-throughput detection for antibody glycosylation.
• The detection is based on chemiluminescent-bead-based lectin binding.
• This method is applicable to evaluation of glycosylation dynamics in cell culture.

We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.