کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2032966 1542885 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of sitosteryl glucoside palmitate in a chloroform-derived fraction of Phyllanthus niruri with antiplasmodial and peripheral antinociceptive properties
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Identification of sitosteryl glucoside palmitate in a chloroform-derived fraction of Phyllanthus niruri with antiplasmodial and peripheral antinociceptive properties
چکیده انگلیسی

ABSTRACTObjectiveTo evaluate the antiplasmodial properties of fractions of chloroform portion of Phyllanthus niruri (P. niruri) methanol extract and identify a suitable chemical marker present therein.MethodsChloroform portion of P. niruri methanol extract was separated from silica gel using gradient systems of hexane, ethylacetate and methanol. The fractions were screened for antiplasmodial activity against Plasmodium falciparum HB3 and FcM29. Fractions with IC50<10 μg/mL against parasites were further screened for peripheral analgesic activity, while cytotoxicity was evaluated using THP-1 cells.ResultsFractions 12-14 were very active (IC50<10 μg/mL) against Plasmodium falciparum and showed no significant cytotoxicity. Fractions 12 and 13 exhibited significant (P<0.01) reduction in acetic acid-induced writhing in mice, decreasing the number of writhes by 66.67% and 65.22% respectively and comparable with 100 mg/kg aspirin (65.22%). From fraction 12, a compound was isolated and identified as sitosteryl-6-β-D-glucoside-6’-palmitate by 1H, 13C nuclear magnetic resonance and mass spectroscopies.ConclusionsOur findings illustrate antiplasmodial column fractions of P. niruri with analgesic activity and identify sitosteryl glucoside palmitate as a chemical marker of activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Biomedicine - Volume 4, Issue 9, September 2014, Pages 707–713
نویسندگان
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