کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2033252 1542894 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antimicrobial and cytotoxic activities of endophytic fungi isolated from Piper crocatum Ruiz & Pav
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Antimicrobial and cytotoxic activities of endophytic fungi isolated from Piper crocatum Ruiz & Pav
چکیده انگلیسی

ABSTRACTObjectiveTo investigate antimicrobial and cytotoxic activities of endophytic fungi isolated from Piper crocatum Ruiz & Pav (P. crocatum).MethodsEthyl acetate extracts were obtained by liquid-liquid partition of fermentation broth of endophytes followed by evaporation. The antimicrobial activities were determined by diffusion techniques followed by thin layer chromatography-bioautography. Cytotoxicity studies were conducted using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Data generated were used to plot a dose-response curve, of which the concentration of extract required to kill 50% of cell population (IC50) was determined.ResultsTwo endophytes were isolated from leaves and stem of P. crocatum, designated as DS1 and BS1. Ethyl acetate extracts of BS1 was found to inhibit the growth of Bacillus subtilis, Escherichia coli and Staphylococcus aureus at minimum dose of 31.25, 125 and 250 µg, respectively. thin layer chromatography-bioautography of this extract resulted in at least two inhibition zones. BS1 extract also inhibited the growth of WiDr and T47D cell lines with IC50 of 120.38 µg/mL and 37.43 µg/mL, respectively.ConclusionsBS1 extract produced by endophytic fungi of P. crocatum is potential to be developed as source of novel bioactive compounds.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Biomedicine - Volume 4, Supplement 2, July 2014, Pages S592–S596
نویسندگان
, , ,