کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
20418 43173 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New enzymatic methods for selective assay of l-lysine using an l-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
New enzymatic methods for selective assay of l-lysine using an l-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395
چکیده انگلیسی

We developed new enzymatic methods for the selective assay of l-lysine by utilizing an oxidase reaction and a decarboxylation reaction by the l-lysine-specific decarboxylase/oxidase (l-Lys-DC/OD) from Burkholderia sp. AIU 395. The method utilizing the oxidase reaction of this enzyme was useful for determination of high concentrations of l-lysine. The method utilizing the decarboxylase reaction, which proceeded via the combination of the l-Lys-DC/OD and putrescine oxidase (PUO) from Micrococcus rubens, was effective for determination of low concentrations of l-lysine. Both methods showed good linearity, and neither was affected by other amino acids or amines. In addition, the within-assay and between-assay precisions of both methods were within the allowable range. The coupling of l-Lys-DC/OD with PUO was also useful for the differential assay of l-lysine and cadaverine. These newly developed methods were applied to the assay of l-lysine in biological samples and found to be effective.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 119, Issue 3, March 2015, Pages 369–374
نویسندگان
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