کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2048349 | 1074077 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Both plant and animal LEA proteins act as kinetic stabilisers of polyglutamine-dependent protein aggregation
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کلمات کلیدی
GST, glutathione transferase - GST، گلوتاتیون ترانسفرازIDP, intrinsically disordered protein - IDP، پروتئین اختلال ذاتی استLEA, late embryogenesis abundant - LEA، زودرس جنین زایی فراوان استpolyQ, polyglutamine - polyQ، polyglutamineThT, Thioflavin T - ThT، Thioflavin TPBS, phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریEGFP, enhanced green fluorescent protein - پروتئین فلورسنت سبز افزایشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Both plant and animal LEA proteins act as kinetic stabilisers of polyglutamine-dependent protein aggregation Both plant and animal LEA proteins act as kinetic stabilisers of polyglutamine-dependent protein aggregation](/preview/png/2048349.png)
چکیده انگلیسی
LEA (late embryogenesis abundant) proteins are intrinsically disordered proteins that contribute to stress tolerance in plants and invertebrates. Here we show that, when both plant and animal LEA proteins are co-expressed in mammalian cells with self-aggregating polyglutamine (polyQ) proteins, they reduce aggregation in a time-dependent fashion, showing more protection at early time points. A similar effect was also observed in vitro, where recombinant LEA proteins were able to slow the rate of polyQ aggregation, but not abolish it altogether. Thus, LEA proteins act as kinetic stabilisers of aggregating proteins, a novel function in protein homeostasis consistent with a proposed role as molecular shields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 585, Issue 4, 18 February 2011, Pages 630–634
Journal: FEBS Letters - Volume 585, Issue 4, 18 February 2011, Pages 630–634
نویسندگان
Yun Liu, Sohini Chakrabortee, Ranhui Li, Yizhi Zheng, Alan Tunnacliffe,